Guidelines for sample preparation (extractions done by users)

Sources of DNA and DNA extraction

We strongly recommend that reference specimens are snap-frozen in the absence of a buffer, and stored at -80°C prior to DNA extraction. For a selection of DNA extraction protocols suited for long-read sequencing, please refer to https://www.protocols.io/groups/awesome-DNA-from-all-kingdoms-of-life and the BioNano homepage

We can process samples using our low-input or express library protocols. The low-input protocol allows construction of PacBio libraries from as little as 5 ng (ultra-low input) or 150 ng (low input) of DNA (recommended for genomes under 300 Mb). For ONT sequencing, we require a minimum DNA amount of 2 ug for each PromethION flow cell. 

All samples are assessed for quality and concentration with the Qubit upon receipt by NGI. 

Please contact olga.pettersson@scilifelab.uu.se for advice on sample collection, tissue preservation and DNA extraction for your specific organism type.