NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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PacBio Sequel

The Scalable Platform for SMRT Sequencing

Image: Courtesy of Pacific Biosciences of California, Inc.

PacBio Sequel System utilizes the Single Molecule, Real Time (SMRT) Sequencing technology which provides high sensitivity, high throughput and fast turnaround times. The core of the Sequel System is the SMRT cells, which contain one million zero-mode waveguides (ZMWs). Active individual polymerases are immobilized within the ZMWs, providing windows to observe and record DNA sequencing in real time.

With longest reads spanning up to 70 Kb, it is suitable for all types of de novo genome sequencing and characterization of a wide variety of genomic variation types, including those in complex regions not accessible with short-read or synthetic long-range sequencing technologies.

Other typical applications include metabarcode studies and pooled amplicons, as sequencing bias toward shorter fragments is low. Since no amplification is required prior to sequencing, methylations in genomic DNA can be detected by analysing interpulse duration times.

Method Status

Service

We are routinely running this method. Please visit the Order Portal to place an order.

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