NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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Illumina lowers the minimum average coverage requirements for HiSeqX to 15X

Illumina has recently updated the Special Conditions of Sale applicable to their HiSeqX instruments. The new Special Conditions lowers the requirements for average depth of coverage and now permits whole genome sequencing (WGS) and bisulphite sequencing down to an average depth of 15X coverage or greater.

The HiSeqX instruments are for many projects at NGI the most affordable Illumina instruments for WGS and bisulphite sequencing since it produces on average over 120 Gb of data per lane. However, many projects have been restricted by the previous conditions that WGS and bisulphite sequencing, of a sample isolated from a single species, had to reach an average depth of coverage of 30X or greater. The new requirements of 15X coverage or greater is therefore a substantial reduction which will make HiSeqX to an even more affordable and attractive alternative for many of our users.

The change applies from January 10 and onwards. Please contact one of our NGI project coordinators for further information and prices.

Details

12th January 2017

By Phil Ewels

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