Environmental sequencing

Methods to determine the species composition of a sample or study genetic variation within a population.

Environmental resequencing
Whole-genome sequencing of microbiota samples.
Amplicon sequencing
Targeted sequencing of e.g. 16S and/or 18S regions, to study the species composition of a sample.
RAD-seq
Discover genetic variants and perform genotyping-by-sequencing without prior genome information.
Illumina 16S sequencing

Sequencing of the 16S gene to study bacterial diversity and composition in a sample.

Illumina amplicon sequencing

Sequencing of PCR amplicons to study genetic variation within small target regions.

Illumina TruSeq DNA PCR-free

Gold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.

Illumina TruSeq DNA Nano

Library preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.

SMARTer ThruPLEX DNA-seq

Library preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.

PacBio SMRT sequencing

PacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.

RAD-sequencing

Genotyping-by-sequencing without prior genome information.

Amplicon-seq analysis

Ampliseq is a bioinformatics analysis pipeline used for 16S rRNA amplicon sequencing data.

PacBio secondary analysis

Analysis applications provided by NGI using PacBio’s open-source SMRT Analysis software suite.

PromethION secondary analysis

Additional compute intensive nanopore raw data processing services provided by NGI

Illumina QC analysis

Basic quality-control monitoring of Illumina FastQ sequence data.

RAD-seq analysis

Pipeline for quality control of Restriction-site Associated DNA sequencing (RAD-Seq). Genotyping-by-sequencing without prior genome information.