High-throughput full-genome or targeted sequencing of DNA from organisms with an existing reference genome.
The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.
library preparation nanoporeSequencing of the 16S gene to study bacterial diversity and composition in a sample.
illumina 16S amplicon targeted library preparationSequencing of PCR amplicons to study genetic variation within small target regions.
illumina 16S amplicon targeted library preparationLow cost library preparation option for gDNA based on bead-linked transposase. Only for full plates of samples.
genome illumina WGS dna nextera normalization library preparationMethod for shotgun DNA libraries used for whole genome sequencing and metagenomics.
genome illumina WGS dna tagmentation PCR-free library preparationGold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.
genome illumina WGS dna library preparation truseqLibrary preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.
genome illumina WGS dna library preparation truseqLibrary preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.
library preparation genome illumina WGS dnaNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
hifi de novo iso seq sv revio smrt assembly pacbio methylation ampliconGenotyping-by-sequencing without prior genome information.
genome illumina wholegenome dna polymorphism library preparationA platform for human whole exome sequencing (WES) using target enrichment and library preparation for next generation sequencing.
library preparation illumina dna exome targetedQuality control, Basecalling and multiplexing of sequencing reads generated by Oxford Nanopore sequencers.
nanopore long-readAdditional compute intensive nanopore raw data processing services provided by NGI
methylation base modifications basecalling pod5Basic quality-control monitoring of Illumina FastQ sequence data.
checkqc QC fastqc fastq screenRuns with illumina DNA-sequencing data, WGS or targeted sequencing e.g. WES. Aligns to the reference genome, gives QC metrics, does variant-calling and finishes with annotation.
WGS sarek cancer human data WES