High-throughput full-genome or targeted sequencing of DNA from organisms with an existing reference genome.
The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.
library preparation nanoporeSequencing of the 16S gene to study bacterial diversity and composition in a sample.
library preparation illumina 16S amplicon targetedSequencing of PCR amplicons to study genetic variation within small target regions.
targeted library preparation illumina 16S ampliconLow cost library preparation option for gDNA based on bead-linked transposase. Only for full plates of samples.
nextera normalization library preparation genome illumina WGS dnaMethod for shotgun DNA libraries used for whole genome sequencing and metagenomics.
PCR-free library preparation genome illumina WGS dna tagmentationGold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.
dna library preparation truseq genome illumina WGSLibrary preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.
WGS dna library preparation truseq genome illuminaLibrary preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.
genome illumina WGS dna library preparationNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
long-read nanopore assemblyPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
smrt assembly pacbio methylation amplicon hifi de novo iso seq sv revioGenotyping-by-sequencing without prior genome information.
wholegenome dna polymorphism library preparation genome illuminaA platform for human whole exome sequencing (WES) using target enrichment and library preparation for next generation sequencing.
library preparation illumina dna exome targetedQuality control, Basecalling and multiplexing of sequencing reads generated by Oxford Nanopore sequencers.
long-read nanoporeAdditional compute intensive nanopore raw data processing services provided by NGI
methylation base modifications basecalling pod5Basic quality-control monitoring of Illumina FastQ sequence data.
QC fastqc fastq screen checkqcRuns with illumina DNA-sequencing data, WGS or targeted sequencing e.g. WES. Aligns to the reference genome, gives QC metrics, does variant-calling and finishes with annotation.
human data WES WGS sarek cancer