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Applications
Technologies

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Applications

De novo sequencing
- Assembly
  - Genome assemblies with HiFi data (Bioinformatics)
  - Illumina DNA (Method)
  - Illumina DNA PCR-Free (Method)
  - Illumina TruSeq DNA PCR-free (Method)
  - Illumina TruSeq DNA Nano (Method)
  - SMARTer ThruPLEX DNA-seq (Method)
  - Nanopore DNA sequencing (Method)
  - PacBio SMRT sequencing (Method)
- Scaffolding
  - Arima HiC (Method)
  - Dovetail Omni-C (Method)
  - Nanopore DNA sequencing (Method)
  - PacBio SMRT sequencing (Method)
- Annotation
  - Illumina TruSeq Stranded mRNA (Method)
  - Nanopore cDNA sequencing (Method)
  - Nanopore Direct RNA sequencing (Method)
  - PacBio SMRT sequencing (Method)
- Genome assemblies with HiFi data (Bioinformatics)
- Nanopore analysis (Bioinformatics)
- PromethION secondary analysis (Bioinformatics)
- Illumina QC analysis (Bioinformatics)
- Nanopore DNA sequencing (Method)
- PacBio SMRT sequencing (Method)
DNA extractions
- HMW DNA extraction (Method)
DNA resequencing
- Whole genome resequencing
  - Illumina DNA (Method)
  - Illumina DNA PCR-Free (Method)
  - Illumina TruSeq DNA PCR-free (Method)
  - Illumina TruSeq DNA Nano (Method)
  - SMARTer ThruPLEX DNA-seq (Method)
  - Nanopore DNA sequencing (Method)
  - PacBio SMRT sequencing (Method)
  - RAD-sequencing (Method)
- Targeted resequencing
  - Ion Torrent secondary analysis (Bioinformatics)
  - Illumina 16S sequencing (Method)
  - Illumina amplicon sequencing (Method)
  - Ion Torrent sequencing (Method)
  - PacBio SMRT sequencing (Method)
  - RAD-sequencing (Method)
- Whole exome sequencing
  - Twist Bioscience Human Exome panel (Method)
- Ion Torrent secondary analysis (Bioinformatics)
- Nanopore analysis (Bioinformatics)
- PromethION secondary analysis (Bioinformatics)
- Illumina QC analysis (Bioinformatics)
- WGS and WES germline / somatic analysis (Bioinformatics)
- Ion Torrent sequencing (Method)
- PacBio SMRT sequencing (Method)
Environmental sequencing
- Environmental resequencing
  - Illumina DNA PCR-Free (Method)
  - Illumina TruSeq DNA PCR-free (Method)
  - Illumina TruSeq DNA Nano (Method)
  - SMARTer ThruPLEX DNA-seq (Method)
  - PacBio SMRT sequencing (Method)
- Amplicon sequencing
  - Amplicon-seq analysis (Bioinformatics)
  - Illumina 16S sequencing (Method)
  - Illumina amplicon sequencing (Method)
  - PacBio SMRT sequencing (Method)
- RAD-seq
  - RAD-sequencing (Method)
- Amplicon-seq analysis (Bioinformatics)
- PromethION secondary analysis (Bioinformatics)
- Illumina QC analysis (Bioinformatics)
- RAD-seq analysis (Bioinformatics)
- PacBio SMRT sequencing (Method)
Epigenetics
- DNA Methylation
  - Enzymatic Methyl-seq (EM-seq) (Method)
  - Methylation-seq analysis (Bioinformatics)
  - SPlinted Ligation Adapter Tagging (SPLAT) for Whole Genome Bisulphite Sequencing (WGBS) (Method)
  - Nanopore DNA sequencing (Method)
  - PacBio SMRT sequencing (Method)
  - Illumina Infinium DNA Methylation EPIC Array (Method)
- ChIP-Sequencing
  - ChIP-seq analysis (Bioinformatics)
  - SMARTer ThruPLEX DNA-seq (Method)
- HiC
  - Arima HiC (Method)
  - Dovetail Omni-C (Method)
- ATAC-Sequencing
  - ATAC-seq analysis (Bioinformatics)
  - Omni-ATAC (Method)
- Enzymatic Methyl-seq (EM-seq) (Method)
- Methylation-seq analysis (Bioinformatics)
- Illumina QC analysis (Bioinformatics)
- SMARTer ThruPLEX DNA-seq (Method)
- Nanopore DNA sequencing (Method)
- PacBio SMRT sequencing (Method)
Genotyping
- Illumina Infinium SNP Genotyping Arrays (Method)
- Illumina Infinium DNA Methylation EPIC Array (Method)
Single-cell
- Smart-seq3 (Method)
- Chromium Single Cell RNA-seq (Method)
- Chromium Single Cell ATAC-seq (Method)
- Chromium Single Cell V(D)J (Method)
- Chromium Feature barcoding / CITE-seq (Method)
Spatial
- Spatial transcriptomics
  - 10X Genomics Visium Fresh Frozen (Method)
  - Spatial Transcriptomics analysis (Bioinformatics)
- 10X Genomics Visium Fresh Frozen (Method)
- Spatial Transcriptomics analysis (Bioinformatics)
Transcriptomics
- Standard short-read RNA-seq
  - Illumina TruSeq Stranded mRNA (Method)
  - Illumina TruSeq Stranded total RNA (RiboZero) (Method)
  - Illumina TruSeq Stranded RNA without selection or depletion (Method)
  - Illumina Stranded total RNA Prep (Ribo-Zero Plus) (Method)
  - RNA-fusion analysis (Bioinformatics)
  - RNA-seq analysis (Bioinformatics)
  - Ion Torrent sequencing (Method)
  - TaKaRa SMARTer pico RNA kit (Method)
- Small RNA
  - Small-RNA analysis (Bioinformatics)
  - QIAseq miRNA low input (Method)
- Full-length transcripts
  - PacBio Iso-Seq Analysis (Bioinformatics)
  - Nanopore cDNA sequencing (Method)
  - Nanopore Direct RNA sequencing (Method)
  - PacBio SMRT sequencing (Method)
- Illumina Stranded total RNA Prep (Ribo-Zero Plus) (Method)
- PacBio Iso-Seq Analysis (Bioinformatics)
- PromethION secondary analysis (Bioinformatics)
- Illumina QC analysis (Bioinformatics)
- RNA-fusion analysis (Bioinformatics)
- Ion Torrent sequencing (Method)
- PacBio SMRT sequencing (Method)
User-prepared libraries
- PromethION secondary analysis (Bioinformatics)
- Illumina QC analysis (Bioinformatics)
- Illumina sequencing (Method)
- Nanopore cDNA sequencing (Method)
- Nanopore DNA sequencing (Method)
- PacBio SMRT sequencing (Method)
Proteomics with NGS readout
- Olink Explore 384-3072 multiplex protein analysis (Method)

Technologies

Last Updated: 31st January 2020

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