NGI can sequence sequencing libraries prepared by research groups.
NGI can sequence user-prepared libraries on all Illumina instruments. User-prepared libraries orders can only be whole-lane orders. Libraries with custom setups and or custom primers can only be whole runs or whole flow cells.
illuminaNanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
assembly long-read nanoporeNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
de novo iso seq sv smrt assembly pacbio methylation amplicon sequel hifi clrAnalysis applications provided by NGI using PacBio’s open-source SMRT Analysis software suite.
base modifications pacbio assembly iso-seq structural variation ccs hifi resequencingAdditional compute intensive nanopore raw data processing services provided by NGI
methylation base modifications basecalling fast5Basic quality-control monitoring of Illumina FastQ sequence data.
QC fastqc fastq screen checkqc