NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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User-prepared libraries

NGI can sequence custom sequencing libraries prepared by research groups.

Illumina sequencing

NGI can sequence user-prepared libraries on all Illumina instruments. User-prepared libraries orders can only be whole-lane orders. Libraries with custom setups and or custom primers can only be whole runs or whole flow cells.

Nanopore cDNA sequencing

Nanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.

Nanopore DNA sequencing

Nanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.

PacBio SMRT sequencing

PacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.

PacBio secondary analysis

Analysis applications provided by NGI using PacBio’s open-source SMRT Analysis software suite.

PromethION secondary analysis

Additional compute intensive nanopore raw data processing services provided by NGI

Illumina QC analysis

Basic quality-control monitoring of Illumina FastQ sequence data.