Price examples

Library preparation for RNA-seq

• Illumina TruSeq Stranded mRNA (with poly-A selection): 1 400 SEK
• Illumina Stranded total RNA Prep (Ribo-Zero Plus) (ribosomal depletion): 2 100 SEK
• Qiagen QIAseq miRNA prep: 1 700 SEK
• TaKaRa SMARTer Pico Input: 1 800 SEK

Please note that these prices do not include the cost of sequencing.
Please note that the RiboZero and SMARTer Pico library preparation methods are only available for certain species, contact us if your samples are from non-mammalian sources.

Library preparations for DNA

• Illumina TruSeq DNA PCR-free library prep: 1 200 SEK
• Illumina DNA PCR-free library prep: 1 200 SEK
• ThruPLEX DNA-Seq library prep: 1 300 SEK
• Illumina DNA noQC: 50 000 SEK (per batch of 94 samples)
• RAD-seq (EcoRI): 32 000 SEK (per batch of 94 samples)

Please note that these prices do not include the cost of sequencing.
Please note that the Illumina DNA noQC and RADseq preps are only run in full plates (94 samples).

Cost of standard RNA-seq

The total cost for library preparation and sequencing depends on the number of samples and the sequencing depth. A general setup, with e.g. 24-96 samples and ~25-50 million read-pairs/sample, will likely cost approx 2000-3000 SEK/sample (incl. library preparation and sequencing on Illumina NovaSeq):
– Library preparation using the Illumina TruSeq Stranded mRNA kit: 96 x ~1 400SEK = ~135 000 SEK
– Sequencing on 1 lane of an Illumina NovaSeq 25B-300 flowcell will generate ~3 billion read-pairs in total, approximately 25 million read-pairs/sample: 35 000 SEK
→ Total cost 170 000 SEK, approximately 2 000 SEK/sample.

Cost for whole-genome re-sequencing (WGS, human genome)

The cost for library preparation and sequencing of a human (or similar size) DNA sample prepared by NGI using the Illumina TruSeq DNA PCR-free library prep or the Illumina DNA PCR-free library prep kit and sequencing on Illumina NovaSeq 25B-300 (2×150 bp) to approximately 30x coverage is approx. 6000 SEK total/sample. Price includes best-practice bioinformatics analysis (currently only available for human genome).

Cost of OmniC

The current cost of one Illumina-compatible OmniC library is approx. 8000 SEK/sample. These libraries are typically sequenced on the Illumina NovaSeq 25B-300 (2×150 bp), but the amount of sequencing that is needed will depend on if the data should be used for e.g. TAD analysis, or scaffolding/polishing of a de novo assembly. A typical setup for assembly scaffolding is approx. 200M reads per 1Gb genome. The total cost of OmniC on one sample is approx. 16 000 SEK (library preparation and sequencing), the cost for two samples likely approx. 25 000 SEK. Price includes a no-guarantees draft scaffolding of an existing assembly.

Please contact us if you need help calculating cost and sequencing requirements for other coverages or genome sizes.

Cost of Amplicon preps (16S or indexing only)

The current cost of running a batch of 94 samples on the 2-PCR-step full 16S library prep is 6500 SEK. The cost of the indexing-only amplicon protocol (you do PCR1, with/without cleanup, we do PCR2) is 4500 SEK/94 samples (you do the cleanup of PCR1) or 5500 SEK/94 samples (we do the PCR2 cleanup).

Up to 384 amplicon libraries can be pooled and sequenced together, typically on the NextSeq 2000 P1-600 flowcell, costing ca 20 000 SEK.

Running 94 samples on the full 16S preps -> 6500 + 20 000 SEK

Running 376 samples on the full 16S preps -> 4×6500 + 20 000 SEK

Cost for Spatial Transcriptomics 10X Visium

The cost to perform a Visium experiment on four Fresh Frozen 6x6mm tissue samples, including an optimisation slide, is approx. 86 000 SEK. Sequencing is usually performed on one lane of an Illumina NovaSeq X Plus 10B-300 flowcell (28-10-10-90) and the cost is approx. 20 000 SEK. The total cost for analysing 4 tissue sections is approx. 106 000 SEK.

The cost to perform one Visium experiments on FF or FFPE samples using the probe-based approach in CytAssist is approx. 95 000 SEK for four 6x6mm tissue samples or 98 000 SEK for two 11x11mm tissue samples. Sequencing is usually performed on one lane of an Illumina NovaSeq X Plus 10B-300 flowcell (28-10-10-50) and the cost is approx. 20 000 SEK. The total cost is 115 000 to 118 000 SEK, depending on the number and size of the samples.

Summary of sequence output and cost

The numbers presented in the table below reflect an average yield based on our observations across projects. The actual yield for different libraries can vary based on library type, concentration of the pool that was loaded on the sequencer, fragment distribution, colour balance of indices, % PhiX spike-in, etc. Please consult with a Project Coordinator for guidance.

Single-lane orders

For NovaSeq X Plus, you can buy single lanes from the 10B-300 and 25B-300 flowcells. This applies only for PE 2x150bp reads, without custom primers. We sell partial (quarter) lanes for 25B-300 only if libraries are prepared by NGI (Stockholm node only).

Please consult with a Project Coordinator if you have any questions about this.