The examples on this page are approximate prices, and are shown here to provide a rough estimate of how much your project will cost. For more detailed information or to request a quote, please contact us.
Our “best practice” bioinformatics package which may be included in some of our services (e.g. RNA-seq, resequencing and de novo assembly) is free of charge. We also do not charge for project planning or follow-up meetings with NGI representatives.
NGI provides very generous pricing for Swedish academia. Instrument depreciations, part of instrument service, rent and labour costs are sponsored by the Swedish Research Council (Vetenskapsrådet) and SciLifeLab. Academic institutions outside of Sweden and users from industry pay the full cost (please contact us for a full-cost price quote). The price estimates on this page are valid only for Swedish academia.
Other methods (e.g. non-validated library preparation types) are available. Please contact us for further information.
Library preparation for RNA-seq
Please note that these prices do not include the cost of sequencing.
Please note that the RiboZero library preparation method is only available for certain species, contact us if your samples are from non-mammalian sources.
Library preparations for DNA
Please note that these prices do not include the cost of sequencing.
Please note that the Nextera Flex noQC and RADseq are only run in full plates.
Cost for standard RNA-seq
The total cost for library preparation and sequencing depends on the number of samples and the sequencing depth. A general setup, with e.g. 24-96 samples and ~25 million read-pairs/sample, will likely cost approx 2000-3000 SEK/sample (incl. library preparation and sequencing on Illumina NovaSeq):
– Library preparation using the Illumina TruSeq Stranded mRNA kit: 24 x ~1 300SEK = ~31 200 SEK, or 96 x ~1 300SEK = ~124 800 SEK
– Sequencing on ¼ – 1 lane of an Illumina NovaSeq S4-300 flowcell will generate ~625 – 2 500 million read-pairs in total, approximately 25 million read-pairs/sample: ~13 000 – 49 000 SEK
→ Total cost ~44 200 – 173 800 SEK, approximately 2 000-3 000 SEK/sample.
Cost for whole-genome re-sequencing (WGS, human genome)
The cost for library preparation and sequencing of a human (or similar size) DNA sample prepared by NGI using the Illumina TruSeq DNA PCR-free library prep kit and sequencing on Illumina NovaSeq S4-300 (2×150 bp) to approximately 30x coverage is approx. 10 000 SEK total/sample. Price includes best-practice bioinformatics analysis (currently only available for human genome).
Cost of OmniC
The current cost of one Illumina-compatible OmniC library is approx. 7900 SEK/sample. These libraries are typically sequenced on the Illumina NovaSeq S4-300 (2×150 bp), but the amount of sequencing that is needed will depend on if the data should be used for e.g. TAD analysis, or scaffolding/polishing of a de novo assembly. A typical setup for assembly scaffolding is approx. 200M reads per 1Gb genome. The total cost of OmniC on one sample is approx. 20 200 SEK (library preparation and sequencing), the cost for two samples likely approx. 33 000 SEK. Price includes a no-guarantees draft scaffolding of an existing assembly.
Please contact us if you need help calculating cost and sequencing requirements for other coverages or genome sizes.
Cost for Spatial Transcriptomics 10X Visium
The cost for one tissue optimisation (TO) experiment is approx. 10 800 SEK. Please have in mind that this step may have to be performed on more than one TO slide. Library preparation (LP) is done in multiples of 4 tissue sections and cost approx. 63 000 SEK per LP slide. Sequencing is usually performed on a Illumina NovaSeq SP-100 flowcell (28-10-10-90 bp) cost approx. 30 000 SEK. The total cost for analysing 4 tissue sections is approx. 100 000 SEK.
For larger projects we also offer sequencing on individual lanes of the Illumina NovaSeq S4-200 flowcell (28-10-10-120 bp), cost approx. 46 200 SEK / lane.
Summary of sequence output and cost
All prices as of 2021-01-31)
One Sequel II cell can produce up to 7 mln single-molecule reads, or up to 500 Gb of data, which effectively corresponds to 1.5 mln HiFi, or up to 30 Gb of unique HiFi bases.
Hence, a bird-size genome is recommended to sequence on 1-2 SMRT cells, a mammalian-size genome on 3-4 SMRT cells.
However, the amount of data produced by the instrument is dependent almost entirely on the DNA quality (link to DNA quality document). The purer and more intact DNA is, the more sequencing data one can get per sequencing unit.
Sequencing price:
One library is enough to sequence on 1-3 SMRT cells.
On PromethION, the amount of data depends on several factors. The main factor is the molecule length. If you opt for sequencing as long reads as possible (up to 1 Mb), the device will rarely produce more than 20 Gb of data.
If you need to get very high throughput, cost-efficient runs, the DNA must be shared to 20 kb, and 50-100 Gb output can be expected.
If one can tolerate slightly lower throughput but want to prioritize longer reads, un-sheared DNA or a sample enriched for DNA molecules in the 75-150kb size range can be used to obtain long- to ultra-long reads, the latter being hundreds of kb up to approximately a million bases long. The human Y-chromosome has been completely assembled from telomere to telomere, a feat not possible without using ultra-long reads.
Sequencing price:
1 ONT library costs c:a 2.5 kSEK
One library is enough for one flow cell only.
Please contact us for detailed information or to request a quote.
The cost for genotyping or array-based methylation profiling varies according to the application and the number of samples. Follow the link below for price details on the most commonly used arrays at NGI:
PriceList_NGI_IlluminaArrays_ValidFrom_Aug1st_2022
Please contact us for detailed information on the different arrays or to request a quote.