NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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Illumina

Illumina supplies a number of instruments for sequencing, primarily through short-read sequencing-by-synthesis and microarray analysis.

At the NGI we have a number of instruments from Illumina depdending on application.

Sequencing:
For sequencing we have a range of Illumina sequencing instruments. All of them work using a “sequencing-by-synthesis” technology, where fluorescent DNA nucleotides are added to a sample one at a time. An imaging system is able to record these flashes of coloured light and covert this information into a linear DNA-sequence.

Illumina sequencing instruments typically sequence relatively short DNA fragments in the order of a few hundred base-pairs. However, they are able to sequence a massive number of these fragments in parallel, giving large quantities of sequence data at competitive prices.

The different Illumina sequencing instruments operate at different scales: the most appropriate depends on the number of samples and the depth of sequencing that you require. The largest machines give the best price per base-pair of DNA but produce a huge volume of data for every run.

Genotyping:
For genotyping we use the iScan system from Illumina, The iScan system is used for genotyping SNPs or DNA methylation analysis of CpG sites by the Infinium assay.

Technologies within this category

iSeq 100
The smallest Illumina sequencing system delivers fast and efficient low-throughput sequencing.
MiSeq
A small scale sequencing instrument ideal for projects that require limited sequencing depth. Suitable for bacterial genomes, and targeted resequencing.
NextSeq 500
A medium scale sequencing instrument ideal for projects that require limited sequencing depth. Suitable for library pools requiring custom read-setups but not requiring as much data being produced on NovaSeq.
NovaSeq 6000
The largest of the Illumina sequencing instruments, able to two flow cells independently of each other and generate massive sequencing depth at competitive prices.
Method Status

Service

We are routinely running this method. Please visit the Order Portal to place an order.

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