Illumina
Illumina supplies a number of instruments for sequencing, primarily through short-read sequencing-by-synthesis, and microarray analysis.
At NGI we have a number of instruments from Illumina, suitable for most applications.
Sequencing
NGI offers sequencing on a range of Illumina sequencing instruments. All of them work using a “sequencing-by-synthesis” technology, where fluorescent DNA nucleotides are added to a sample one at a time. An imaging system records these flashes of coloured light and converts this information into a linear DNA-sequence.
Illumina sequencing instruments typically sequence relatively short DNA fragments, on the order of a few hundred base-pairs. They are, however, able to sequence a massive number of these fragments in parallel, giving large quantities of sequence data at competitive prices.
The different Illumina sequencing instruments operate at different scales – the most appropriate one for your project depends on the number of samples you have and the depth of sequencing that you require. The largest machines give the best price per base-pair of DNA but produce a very large volume of data in each run.
Genotyping
For genotyping we use the iScan system from Illumina, The iScan system is used for genotyping SNPs or DNA methylation analysis of CpG sites by the Infinium assay. Please visit our page for Genotyping for more information.
Last Updated: 3rd October 2024