Illumina supplies a number of instruments for sequencing, primarily through short-read sequencing-by-synthesis and microarray analysis.
At the NGI we have a number of instruments from Illumina depdending on application.
For sequencing we have a range of Illumina sequencing instruments. All of them work using a “sequencing-by-synthesis” technology, where fluorescent DNA nucleotides are added to a sample one at a time. An imaging system is able to record these flashes of coloured light and covert this information into a linear DNA-sequence.
Illumina sequencing instruments typically sequence relatively short DNA fragments in the order of a few hundred base-pairs. However, they are able to sequence a massive number of these fragments in parallel, giving large quantities of sequence data at competitive prices.
The different Illumina sequencing instruments operate at different scales: the most appropriate depends on the number of samples and the depth of sequencing that you require. The largest machines give the best price per base-pair of DNA but produce a huge volume of data for every run.
For genotyping we use the iScan system from Illumina, The iScan system is used for genotyping SNPs or DNA methylation analysis of CpG sites by the Infinium assay.