News » Research & Development

The NGI runs internal research & development projects to establish new methods, benchmark techniques, and test new vendor kits. Where we believe this work to be of general interest to the scientific community, we make it open source.

Tech Note РAdaptive Sampling: targeted  Oxford Nanopore long-read sequencing

29th February 2024  -  Categories: Research & Development

Adaptive sampling can be used to selectively enrich or deplete sequences from a library in real-time, enhancing coverage of regions of interest without requiring additional library pre-processing

Tech Note: SARS-CoV-2 Illumina Sequencing

10th February 2021  -  Categories: Research & Development

In order to provide genome sequencing of SARS-CoV-2 samples for research groups working with the NGI, we tested a new commercially available kit using multiplexed amplicons.

Tech Note: Increased Complexity of Amplicon Libraries using Phased Primers

13th January 2021  -  Categories: Research & Development

Phased primers are used during the first PCR of amplicon library preparation in order to increase the complexity of the library. This increased complexity leads to improved sequencing quality.

Tech Note: Simplified protocol for SMARTer Pico kit

17th August 2020  -  Categories: Research & Development

Is total RNA fragmentation needed? Is rRNA depletion affected by fragmentation time? Or affected by input? Can we get reliable data when starting with low input?