The NGI runs internal research & development projects to establish new methods, benchmark techniques, and test new vendor kits. Where we believe this work to be of general interest to the scientific community, we make it open source.
10th February 2021 - Categories: Research & Development
In order to provide genome sequencing of SARS-CoV-2 samples for research groups working with the NGI, we tested a new commercially available kit using multiplexed amplicons.
13th January 2021 - Categories: Research & Development
Phased primers are used during the first PCR of amplicon library preparation in order to increase the complexity of the library. This increased complexity leads to improved sequencing quality.
17th August 2020 - Categories: Research & Development
Is total RNA fragmentation needed? Is rRNA depletion affected by fragmentation time? Or affected by input? Can we get reliable data when starting with low input?