Hands-on Course in Epigenomics
The Hands-on Course in Epigenomics is a four-day intensive course combining laboratory work, theory, and bioinformatics training, focused on the generation and analysis of DNA methylation data.
This four-day practical course in epigenomics integrates lab experiments, theoretical sessions, and bioinformatics, with an emphasis on generating and analyzing DNA methylation data. The course will be held at SciLifeLab/BMC in Uppsala, where the laboratory-based training will be conducted in the NGI OpenLab.
Course agenda
November 11-12th Hands-on and theoretical training in WGBS and short-read sequencing.
November 13th Hands-on and theoretical training in long-read WGS.
November 14th Best practices for data analysis.
Each student will receive reagents to prepare up to 6 whole genome bisulfite sequencing (WGBS) libraries. Students are welcome to bring their own DNA samples or to use control DNA from cell lines provided by the organizers.
Lunch and fika will be provided.
This course is part of the SciLifeLab Epigenomics Symposium, which takes place on November 10th. All course attendees are encouraged to attend the Symposium. Requires separate sign-up.
Due to limited bench space, the course can accommodate only 8-10 participants. All applicants will be notified of their acceptance by October 10, 2025.
Prerequisites
- Laboratory skills: Participants should have prior hands-on experience with basic molecular biology techniques. At a minimum, you should be comfortable with pipetting and have a basic understanding of PCR.
- Bioinformatics: No previous experience is required.
Learning outcomes
By the end of the course, participants will be able to:
- Explain the principles of NGS library construction
- Prepare WGBS libraries using SPLAT (Raine et al., NAR 2017)
- Perform library quality control using standard methods
- Understand how to start sequencing runs on Illumina MiSeq i100 and ONT MinION platforms
- Apply best practices for DNA methylation data quality control and analysis
- Navigate the process for submitting sequencing libraries to NGI
Target audience: research engineers, PhD Students, postdocs, researchers
Tech requirements: Course participants are required to bring a laptop for the bioinformatics module. Other than that, no other requirements. Instrumentation and materials for the wet-lab course will be provided.
Cost: 2500 SEK
Contact: Ulrika Liljedahl (ulrika.liljedahl@medsci.uu.se)
