The 10X Genomics Chromium single cell gene expression protocols allow one to look at gene expression (GE) in thousands of cells from each sample. The Chromium setup is based on a single cell/nucleus suspension being pushed through a channel, where it meets up with a 10X barcoded gel bead. This results in the formation of a unique GEM partition – an oil bubble where RT and cDNA synthesis is performed – and all cDNA from a particular GEM will share the same 10X barcodes. An Illumina-compatible library is made from the cDNA and sequencing is typically performed on the Illumina NextSeq or NovaSeq platforms.
Last Updated: 16th May 2024