NGI webinar on de novo sequencing and analysis

NGI welcomes you to a webinar about de novo sequencing and analysis.

Welcome to a webinar on de novo sequencing and analysis, a service that the National Genomics Infrastructure (NGI) and National Bioinformatics Infrastructure Sweden (NBIS) offer to their users. De novo, which translates to “starting from the beginning”, refers to the process of sequencing a novel genome from an organism where no previous reference sequence is available.

The de novo analysis strategy is divided into separate stages, starting with
(i) sequencing,
(ii) assembly, and finally
(iii) annotation.

During the sequencing stage, short and/or long DNA/RNA reads are produced using several sequencing technologies, such as Illumina, PacBio and Nanopore. The obtained DNA reads are subsequently assembled into longer sequences, called contigs during the assembly stage. The gaps between contigs can be linked by scaffolding using long read data and chromosome contact maps obtained by Hi-C.Assembled genomes are then annotated, allowing for the structure and the function of the assembled sequences to be inferred. This includes protein-coding genes, but also other features such as non-coding genes and regulatory elements. This webinar will focus on what one needs to consider while planning a de novo project. We are also proud to have Alexander Suh (Lecturer, University of East Anglia and Uppsala University) describing one of his many projects on how to solve sex-specific and tissue-specific genome differences of birds by using de novo tools facilitated by NGI and NBIS.

Date and time: May 26, 10:00-12:00

Please register here.



10:00 NGI webinar on de novo sequencing and analysis
Olga Vinnere Pettersson, PhD, Project coordinator NGI

10:20 PacBio/Nanopore in de novo realm
Ignas Bunikis, PhD, Bioinformatician NGI

10:35 Using Hi-C methods to generate chromosome-scaled assembly scaffolds
Remi-Andre Olsen, PhD, Bioinformatician NGI

10:50 The assembly and annotation support available at the SciLifeLab bioinformatics platform NBIS
Henrik Lantz, PhD, Joint Head of Facility Support and Infrastructure NBIS

11:05 Diving into the depths of sex-specific and tissue-specific genome differences of birds
Alexander Suh, PhD, Lecturer, University of East Anglia and Uppsala University

11:30 Q&A session

Date / Time


10:00 - 12:00

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