In split-pool barcoding methods, single-cell identity is generated through successive rounds of distributing fixed cells across plate wells, where each round introduces a distinct barcode. After each barcoding step, cells are pooled and redistributed, and the final combination of barcodes uniquely labels the cDNAs from each individual cell.
The Parse Biosciences Evercode™ split-pool combinatorial barcoding system involves multiple rounds of barcoding of fixed and permeabilised cells.
Last Updated: 29th January 2026