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Tech Note: Increased Complexity of Amplicon Libraries using Phased Primers

13th January 2021  -  Categories: Research & Development

Phased primers are used during the first PCR of amplicon library preparation in order to increase the complexity of the library. This increased complexity leads to improved sequencing quality.

Tech Note: Simplified protocol for SMARTer Pico kit

17th August 2020  -  Categories: Research & Development

Is total RNA fragmentation needed? Is rRNA depletion affected by fragmentation time? Or affected by input? Can we get reliable data when starting with low input?