Runs with illumina RNA-Seq data. Aligns to the reference genome, gives QC metrics, predicted gene fusions and finishes with graphically visualised reports.
rnafusion is a bioinformatics best-practice analysis pipeline used on RNA-Seq data with the intention of localising and visualising fusion genes.
The pipeline uses Nextflow, a bioinformatics workflow tool. It pre-processes raw data from FastQ inputs, aligns the reads and performs extensive quality-control on the results.
The workflow is built on a list of curated tools such as Arriba, EricScript, and FusionCatcher.
When we run analysis
We run this analysis for RNA-seq projects where we have prepared the sequencing library in-house. If you have prepared a library yourself and we are just sequencing, please get in touch and mention that you would like us to run this analysis.
The analysis works with any of the species that have a reference genome available in AWS-iGenomes. If in doubt, please ask whether we can run the pipeline for you.
bcl2fastq demultiplexed FastQ files and a genome reference.
Depending on the tools specified, the pipeline generates text files with all the predicted gene fusions, BAM-alignment files and visualised reports in HTML-format.
Last Updated: 24th March 2022