Investigate transcription levels and isoforms, e.g. for studies of differential expression.
Sequence your total RNA and choose to perform an rRNA and/or globin RNA depletion. This procedure will allow to sequence long non-coding RNA and other non-polyadenylated RNAs, in addition to mRNAs. We offer this service using the Illumina TruSeq Stranded total RNA kit, as well as the Takara SMARTer Stranded Total RNA-Seq Kit (non-validated protocol) for low-input total RNA samples. Please note that NGI will normally not perform RNA extraction for users. Please contact us for discussions.