Gene expression

Investigate transcription levels and isoforms, e.g. for studies of differential expression.

Sequence your total RNA and choose to perform an rRNA and/or globin RNA depletion. This procedure will allow to sequence long non-coding RNA and other non-polyadenylated RNAs, in addition to mRNAs. We offer this service using the Illumina TruSeq Stranded total RNA kit, as well as the Takara SMARTer Stranded Total RNA-Seq Kit (non-validated protocol) for low-input total RNA samples. Please note that NGI will normally not perform RNA extraction for users. Please contact us for discussions.

Methods 8 Bioinformatics 2

Illumina TruSeq Stranded mRNA

RNA sequencing of mRNAs selected through poly-A enrichment.

library preparation transcriptomics RNA truseq mRNA illumina RNA-Seq

Illumina TruSeq Stranded total RNA (RiboZero)

RNA sequencing of all RNA in a sample after depletion of rRNA or another type of highly abundant RNA.

library preparation transcriptomics RNA truseq mRNA illumina RNA-Seq

Illumina TruSeq Stranded RNA without selection or depletion

RNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.

library preparation RNA truseq illumina rnaseq RNA-Seq

Chromium Single Cell RNA-seq

Profiling of gene expression levels at single-cell resolution.

library preparation transcriptomics single cell RNA mRNA illumina RNA-Seq chromium 10x Genomics

Ion Torrent sequencing

Ion Torrent semiconductor sequencing technology is as simple as it is fast.

ion torrent s5 amplicon ampliseq

Nanopore cDNA sequencing

Nanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.

assembly long-read nanopore

Nanopore Direct RNA sequencing

Nanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.

assembly long-read nanopore

TaKaRa SMARTer pico RNA kit

The Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit is specifically designed for very low input total RNA samples. It also works with degraded total RNA.

library preparation transcriptomics RNA illumina rnaseq RNA-Seq depletion

RNA-fusion analysis

Runs with illumina RNA-Seq data. Aligns to the reference genome, gives QC metrics, predicted gene fusions and finishes with graphically visualised reports.

fusion rnafusion

RNA-seq analysis

Runs with illumina total RNA-sequencing data. Aligns to the reference genome, gives QC metrics and finishes with gene count matrices.

transcriptomics rnaseq RNA-Seq