Investigate transcription levels and isoforms, e.g. for studies of differential expression.
Sequence your total RNA and choose to perform an rRNA and/or globin RNA depletion. This procedure will allow to sequence long non-coding RNA and other non-polyadenylated RNAs, in addition to mRNAs. We offer this service using the Illumina TruSeq Stranded total RNA kit, as well as the Takara SMARTer Stranded Total RNA-Seq Kit (non-validated protocol) for low-input total RNA samples. Please note that NGI will normally not perform RNA extraction for users. Please contact us for discussions.
RNA sequencing of mRNAs selected through poly-A enrichment.
library preparation transcriptomics RNA truseq mRNA illumina RNA-SeqRNA sequencing of all RNA in a sample after depletion of rRNA or another type of highly abundant RNA.
library preparation transcriptomics RNA truseq mRNA illumina RNA-SeqRNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
library preparation RNA truseq illumina rnaseq RNA-SeqProfiling of gene expression levels at single-cell resolution.
library preparation transcriptomics single cell RNA mRNA illumina RNA-Seq chromium 10x GenomicsIon Torrent semiconductor sequencing technology is as simple as it is fast.
ion torrent s5 amplicon ampliseqNanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
assembly long-read nanoporeNanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.
assembly long-read nanoporeThe Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit is specifically designed for very low input total RNA samples. It also works with degraded total RNA.
library preparation transcriptomics RNA illumina rnaseq RNA-Seq depletionRuns with illumina RNA-Seq data. Aligns to the reference genome, gives QC metrics, predicted gene fusions and finishes with graphically visualised reports.
fusion rnafusionRuns with illumina total RNA-sequencing data. Aligns to the reference genome, gives QC metrics and finishes with gene count matrices.
transcriptomics rnaseq RNA-Seq