Multiple rounds of barcoding of fixed and permeabilised cells.
parse combinatorial transcriptomics RNA mRNA illumina RNA-Seq single-cellNEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
WGBS EM-seq library preparation methylation epigenetics illumina CpGPoly(A)-enrichment mRNA sequencing specifically isolates polyadenylated transcripts from total RNA to enable focused, strand-specific profiling of protein-coding genes, isoforms, and structural variations.
library preparation transcriptomics RNA mRNA illumina RNA-SeqRNA sequencing of mRNAs selected through poly-A enrichment.
RNA truseq mRNA illumina RNA-Seq library preparation transcriptomicsRNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
RNA truseq illumina rnaseq RNA-Seq library preparationTotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
illumina RNA-Seq library preparation transcriptomics RNA mRNANGI offers sequencing on a number of instruments from Illumina, suitable for most applications. NGI also runs genotyping using the Illumina iScan system.
illumina short reads