Multiple rounds of barcoding of fixed and permeabilised cells.
transcriptomics RNA mRNA illumina RNA-Seq single-cell parse combinatorialNEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
library preparation methylation epigenetics illumina CpG WGBS EM-seqPoly(A)-enrichment mRNA sequencing specifically isolates polyadenylated transcripts from total RNA to enable focused, strand-specific profiling of protein-coding genes, isoforms, and structural variations.
RNA mRNA illumina RNA-Seq library preparation transcriptomicsRNA sequencing of mRNAs selected through poly-A enrichment.
illumina RNA-Seq library preparation transcriptomics RNA truseq mRNARNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
RNA-Seq library preparation RNA truseq illumina rnaseqTotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
library preparation transcriptomics RNA mRNA illumina RNA-SeqNGI offers sequencing on a number of instruments from Illumina, suitable for most applications. NGI also runs genotyping using the Illumina iScan system.
illumina short reads