Pacific Biosciences
Pacific Biosciences Single Molecule, Real-Time (SMRT) Sequencing technology provides the most comprehensive view of genomes, transcriptomes, and epigenomes.
Image: Courtesy of Pacific Biosciences of California, Inc.
SMRT sequencing technology generates reads tens of kilobases in length allowing to assemble complete genomes and sequence full-length transcripts. Technology is free of systematic errors and achieves >99.999% consensus accuracy. No bias based on GC content means sequencing through regions inaccessible to other technologies. Capturing sequence data from native DNA or RNA molecules enables highly accurate long reads with >99% single-molecule accuracy. With no PCR amplification step, base modifications are directly detected during sequencing.
To learn more about technology, visit Pacific Biosciences site.
NGI offers de novo sequencing using the Revio system
Reference genomes are currently commonly assembled de novo using only long-read technologies because it results in long contiguous assemblies (contig N50 over 1 Mb) with a few scaffolds.
For a good reference-quality assembly, one should aim for 20x (minimum 15x) of effective coverage per allele with HiFi reads. NGI currently recommends PacBio for production of the bulk sequencing data. For data consisting of a high number of PacBio HiFi reads, no additional polishing with Illumina is required, except for HiC/OmniC for scaffolding.
Last Updated: 11th September 2024