NovaSeq 6000
High throughput Illumina sequencing instruments.
Library pools can be sequenced on either whole flow cells or on individual lanes. Please note that we typically only sell full NovaSeq 6000 flowcells.
The selection of different flow cells can be seen in the table below. The choice of flow cell will depend on a number of factors:
- The amount of data needed per sample
- Number of available barcodes for the chosen library preparation method
- Nature of the libraries, such as fragment length, requirement for custom primers, etc.
- Required read-length & if custom sequencing primers are required
NGI coordinators will help you choose the best option for your project if you are unsure.
Flow cell | Lanes | Yield (clusters/lane) | Read length | |
---|---|---|---|---|
SP | 2 | Minimum: ≥325M Typical: 500M | 100 cycles (e.g. 2x50bp) 200 cycles (e.g. 2x100bp) 300 cycles (e.g. 2x150bp) 500 cycles (e.g. 2x250bp) | |
S1 | 2 | Minimum: ≥650M Typical: 900M | 100 cycles (e.g. 2x50bp) 200 cycles (e.g. 2x100bp) 300 cycles (e.g. 2x150bp) | |
S2 | 2 | Minimum: ≥1650M Typical: 2000M | 100 cycles (e.g. 2x50bp) 200 cycles (e.g. 2x100bp) 300 cycles (e.g. 2x150bp) | |
S4 | 4 | Minimum: ≥2000M Typical: 3000M | 200 cycles (e.g. 2x100bp) 300 cycles (e.g. 2x150bp) |
Quality scores:
- Greater than 85% of bases above Q30 at 2 × 50 bp
- Greater than 80% of bases above Q30 at 2 × 100 bp
- Greater than 75% of bases above Q30 at 2 × 150 bp
Minimum yield in the table above is what we guarantee if the libraries sequenced have passed our quality controls. Typical yield is based on what we get from most of the common library types made by us, but this can vary from case to case.
There will be a certain variation in yield between samples in a library pool depending on variations in pipetting and concentration measurements. NGI will accept a variation of +/- 25% between samples in a pool.
- Pools with libraries not made by NGI always need to be sequenced on whole lanes
- Libraries needing custom sequence and/or index primers need to be sequenced on whole flow cells
- Libraries needing non-standard read setups may need to be sequenced on whole flow cells
Last Updated: 22nd January 2024