NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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Small RNA

Assess the abundance and identity of different small-RNA such as miRNA, directly from total RNA.

Sequence miRNA and small RNA directly from total RNA, for any species. NGI offers this service using the Illumina TruSeq Small RNA kit, with the possibility to pool up to 48 samples using single indexes. Additionally, we are currently working on establishing a new protocol for low input samples using the QIAseq miRNA Library Kit.

Illumina TruSeq Small RNA

Generation of sequencing libraries from dicer cleaved miRNA.

QIAseq miRNA low input

Generation of miRNA libraries from very low input total RNA samples and degraded total RNA.

Small-RNA analysis

Runs with illumina small RNA-sequencing data. Aligns to the reference genome, gives QC metrics and finishes with gene count matrices.