Assess the abundance and identity of different small-RNA such as miRNA, directly from total RNA.
Sequence miRNA and small RNA directly from total RNA, for any species. NGI offers this service using the Illumina TruSeq Small RNA kit, with the possibility to pool up to 48 samples using single indexes. Additionally, we are currently working on establishing a new protocol for low input samples using the QIAseq miRNA Library Kit.
Generation of sequencing libraries from dicer cleaved miRNA.
truseq illumina library preparation epigenetics RNAGeneration of miRNA libraries from very low input total RNA samples and degraded total RNA.
library preparation epigenetics RNA illuminaRuns with illumina small RNA-sequencing data. Aligns to the reference genome, gives QC metrics and finishes with gene count matrices.
smrnaseq small RNA smrna-seq