NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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Associated Keywords: epigenetics

Arima HiC

Production of high-quality proximity ligation libraries, using two restriction enzymes.

ATAC-seq

A method to identify open chromatin regions, such as promotor areas, using a transposase.

Dovetail Omni-C

A proximity-ligation protocol using a sequence-independent endonuclease, generating data for TAD identification and scaffolding.

SPlinted Ligation Adapter Tagging (SPLAT) for Whole Genome Bisulphite Sequencing (WGBS)

SPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNA

Illumina TruSeq Small RNA

Generation of sequencing libraries from dicer cleaved miRNA.

QIAseq miRNA low input

Generation of miRNA libraries from very low input total RNA samples and degraded total RNA.

Illumina Infinium DNA Methylation EPIC Array

The EPIC BeadChip array allows for the interrogation over 850,000 methylation sites quantitatively across the genome at single-nucleotide resolution.