NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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Associated Keywords: library preparation

Illumina TruSeq Stranded mRNA

RNA sequencing of mRNAs selected through poly-A enrichment.

Illumina TruSeq Stranded total RNA (RiboZero)

RNA sequencing of all RNA in a sample after depletion of rRNA or another type of highly abundant RNA.

Illumina TruSeq Stranded RNA without selection or depletion

RNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.

Arima HiC

Production of high-quality proximity ligation libraries, using two restriction enzymes.

Chromium Single Cell V(D)J

Simultaneous analysis of gene expression and immunoprofiling of V(D)J clonotypes in the same single cell.

Chromium Feature barcoding / CITE-seq

Multimodal single cell phenotyping method that enables simultaneous analysis of the transcriptome as well as the proteome of single cells.

Nadia instrument (Dolomite Bio)

Nadia takes scRNA-Seq to the next level by using automation and flexibility to generate high quality reproducible single cell data.

Dovetail Omni-C

A proximity-ligation protocol using a sequence-independent endonuclease, generating data for TAD identification and scaffolding.