The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.
library preparation nanoporeProfiling of gene expression levels at single-cell resolution.
single-cell library preparation transcriptomics single cell illumina rnaseq smartseq3 SS3NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
CpG WGBS EM-seq library preparation methylation epigenetics illuminaRNA sequencing of mRNAs selected through poly-A enrichment.
illumina RNA-Seq library preparation transcriptomics RNA truseq mRNARNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
rnaseq RNA-Seq library preparation RNA truseq illuminaTotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
illumina RNA-Seq library preparation transcriptomics RNA mRNASingle-cell profiling of gene expression levels on a large number of cells.
mRNA illumina RNA-Seq scale library preparation transcriptomics single cell RNACombining short & long reads at the single cell level.
chromium 10x Genomics full transcript library preparation single cell long-read illumina ontProfiling of chromatin accessibility at the single cell level.
ATAC library preparation epigenetics single cell illumina ATAC-seq dna chromium chromatin 10x GenomicsProfiling of 3´gene expression and chromatin accessibility in the same cell.
mRNA illumina ATAC-seq RNA-Seq ATAC chromium library preparation 10x Genomics transcriptomics single cell RNA