The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.
library preparation nanoporeProfiling of gene expression levels at single-cell resolution.
rnaseq smartseq3 SS3 single-cell library preparation transcriptomics single cell illuminaNEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
epigenetics illumina CpG WGBS EM-seq library preparation methylationRNA sequencing of mRNAs selected through poly-A enrichment.
truseq mRNA illumina RNA-Seq library preparation transcriptomics RNARNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
truseq illumina rnaseq RNA-Seq library preparation RNATotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
mRNA illumina RNA-Seq library preparation transcriptomics RNAProduction of high-quality proximity ligation libraries, using two restriction enzymes.
library preparation epigenetics TADs illumina de novo chromatin scaffoldingSingle-cell profiling of gene expression levels on a large number of cells.
library preparation transcriptomics single cell RNA mRNA illumina RNA-Seq scaleCombining short & long reads at the single cell level.
single cell long-read illumina ont chromium 10x Genomics full transcript library preparationProfiling of chromatin accessibility at the single cell level.
single cell illumina ATAC-seq dna chromium chromatin 10x Genomics ATAC library preparation epigenetics