NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
library preparation methylation epigenetics illumina CpG WGBS EM-seqRNA sequencing of mRNAs selected through poly-A enrichment.
library preparation transcriptomics RNA truseq mRNA illumina RNA-SeqRNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
library preparation RNA truseq illumina rnaseq RNA-SeqTotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
library preparation transcriptomics RNA mRNA illumina RNA-SeqCombining short & long reads at the single cell level.
single cell long-read illumina ont chromium 10x Genomics full transcript library preparationProfiling of chromatin accessibility at the single cell level.
illumina ATAC-seq dna chromium chromatin 10x Genomics ATAC library preparation epigenetics single cellProfiling of 3´gene expression and chromatin accessibility in the same cell.
ATAC chromium library preparation 10x Genomics transcriptomics single cell RNA mRNA illumina ATAC-seq RNA-SeqProfiling of gene expression levels at single-cell resolution.
RNA mRNA illumina RNA-Seq chromium 10x Genomics library preparation transcriptomics single cellSingle-cell profiling of gene expression levels on fixed cells/nuclei.
transcriptomics single cell multiplexing RNA probes mRNA FFPE illumina RNA-Seq sequencing chromium 10x Genomics library preparation fixed cells