Nanopore sequencing

NGI can sequence user-prepared libraries on Oxford Nanopore PromethION. User-prepared libraries orders can only be sequenced on whole flowcells.

Sample requirements

We need ca 50-100 fmol of the library in exactly 32 uL.

Please also let us know what kit has been used.

Libraries need to be shipped on dry ice and stored in -80.

What we do with your samples

We will not do any reception control, the whole volume will be loaded directly on the flowcell so it’s important that you make sure you prepare a suitable concentration based on the fragment size distribution in your library.

Last Updated: 28th May 2026

Please read our sample submission instructions before sending samples:

Sample Submission Guidelines
Applications
Relevant Technologies
Bioinformatics Pipelines
Method Status

Service

We are routinely running this method. Please visit the Order Portal to place an order.

NGI

This protocol is available at both NGI nodes.

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