This protocol is available at both NGI nodes.
RNA sequencing of mRNAs selected through poly-A enrichment.
RNA truseq mRNA illumina RNA-Seq library preparation transcriptomicsRNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
truseq illumina rnaseq RNA-Seq library preparation RNANGI can sequence user-prepared libraries on Oxford Nanopore PromethION. User-prepared libraries orders can only be sequenced on whole flowcells.
nanoporeNGI can sequence user-prepared libraries on all Illumina instruments. User-prepared libraries orders can only be whole-lane orders. Libraries with custom setups and or custom primers can only be whole flow cells.
illuminaLibrary preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.
genome illumina WGS dna library preparationNanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
long-read nanopore assemblyNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
assembly long-read nanoporeNGI offers sequencing on a number of instruments from Illumina, suitable for most applications. NGI also runs genotyping using the Illumina iScan system.
illumina short readsBasic quality-control monitoring of Illumina FastQ sequence data.
QC fastqc fastq screen checkqc