MiSeq i100 Plus
The Illumina MiSeq i100 Plus is a compact, high-throughput bench-top sequencer available at the NGI Openlab and as a standard service at NGI Stockholm.

Yield: 1.5–30 Gb per run
Reads per run: 5-100 M read-pairs
Read lengths: up to 2 x 500 bases

MiSeq i100 Plus cartridges include both the reagents and the flow cell, enabling easy, independent sequencing.
Fast and flexible sequencing: Short run times, scalable output, and exceptional data quality.
The MiSeq i100 Plus is available as a standard sequencing service at NGI Stockholm, but is is also available for use at NGI OpenLab, meaning researchers perform their own sequencing independently. When sequencing through the OpenLab, reagents are purchased separately, with 5M and 25M kits currently available; for details on available kits, read lengths, and upcoming options, please visit Illumina or the NGI OpenLab homepage.
Key Features
- Fast Run Times: Achieve results in as little as 4 hours.
- High Data Quality: >90% of bases above Q30.
- Flexible Output: 1.5–30 Gb per run, supporting up to 100 million read-pairs.
- User-Friendly Interface: Intuitive touchscreen operation with onboard analysis.
- Room-Temperature Reagents: Eliminates the need for reagent thawing, expediting workflow.
- “Long short reads”: Up to 2 x 500 bases.
Applications
The MiSeq i100 Plus is suitable for a wide range of applications, including:
- Targeted Gene Sequencing
- 16S Metagenomic Sequencing
- Small Genome Sequencing
- Gene Expression Profiling
- Amplicon Sequencing
Last Updated: 17th February 2026