The NGI runs internal research & development projects to establish new methods, benchmark techniques, and test new vendor kits. Where we believe this work to be of general interest to the scientific community, we make it open source.
22nd May 2024 - Categories: Research & Development
A detailed characterization of the REH cell line using combination of short- and long-read DNA and RNA sequencing.
20th May 2024 - Categories: Research & Development
Covaris g-Tubes can be used to fragment DNA and are recommended for use in creating Oxford Nanopore (ONT) libraries. The volumes and DNA concentration typically used for ONT libraries are outside the range of the example data provided by Covaris. Here, we tested using the volumes and concentrations appropriate for our requirements.
29th February 2024 - Categories: Research & Development
Adaptive sampling can be used to selectively enrich or deplete sequences from a library in real-time, enhancing coverage of regions of interest without requiring additional library pre-processing
10th October 2023 - Categories: Research & Development
Open-source genomic, transcriptomic and epigenomic data sets (raw sequencing reads as well as assemblies and mapped BAM files) for a leukemic cell line (REH), available to the research community.
24th August 2023 - Categories: Research & Development
Showing the promise of long-read sequencing toward characterization of the full spectrum of genetic variation in single cells.
6th April 2022 - Categories: Research & Development
Introducing single cell Splinted Ligation Adapter Tagging (scSPLAT), a scalable plate-based protocol for single-cell WGBS library preparation, offering sample preparation at a higher scale and throughput.
10th February 2021 - Categories: Research & Development
In order to provide genome sequencing of SARS-CoV-2 samples for research groups working with the NGI, we tested a new commercially available kit using multiplexed amplicons.
13th January 2021 - Categories: Research & Development
Phased primers are used during the first PCR of amplicon library preparation in order to increase the complexity of the library. This increased complexity leads to improved sequencing quality.
4th September 2020 - Categories: Research & Development
Sarek: an open-source bioinformatic tool to detect germline variants and somatic mutations based on sequencing data from WGS, whole-exome sequencing (WES), or gene panels. Sarek offers easy, efficient, and reproducible analyses, and can readily be used both as a production workflow at sequencing facilities and as a powerful stand-alone tool for individual research groups.
17th August 2020 - Categories: Research & Development
Is total RNA fragmentation needed? Is rRNA depletion affected by fragmentation time? Or affected by input? Can we get reliable data when starting with low input?