NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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Associated Keywords: WGS

Illumina Nextera DNA Flex

Low cost library preparation option for gDNA based on bead-linked transposase. Only for full plates of samples.

Illumina TruSeq DNA PCR-free

Gold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.

Illumina TruSeq DNA Nano

Library preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.

SMARTer ThruPLEX DNA-seq

Library preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.

TELL-seq

Library preparation technology linking reads from long DNA fragments useful for example for de novo sequencing or phasing of variants in whole genome resequencing.

WGS analysis

Runs with illumina human DNA-sequencing data. This goes through alignment, alignment quality control, data processing, variant calling, and variant filtration.

WGS germline / somatic analysis

Runs with illumina DNA-sequencing data, WGS or targeted sequencing. Aligns to the reference genome, gives QC metrics, does variant-calling and finishes with annotation.