NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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Amplicon sequencing

Targeted sequencing of e.g. 16S and/or 18S regions, to study the species composition of a sample.

Illumina 16S sequencing

Sequencing of the 16S gene to study bacterial diversity and composition in a sample.

Illumina amplicon sequencing

Sequencing of PCR amplicons to study genetic variation within small target regions.

PacBio SMRT sequencing

PacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.

Amplicon-seq analysis

Ampliseq is a bioinformatics analysis pipeline used for 16S rRNA amplicon sequencing data.

PacBio secondary analysis

Analysis applications provided by NGI using PacBio’s open-source SMRT Analysis software suite.