NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
EM-seq library preparation methylation epigenetics illumina CpG WGBSSPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNA
epigenetics illumina CpG WGBS Bisulphite single-stranded DNA ssDNA library preparation methylationPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
sv revio smrt assembly pacbio methylation amplicon sequel hifi clr de novo iso seqThe EPIC BeadChip array allows for the interrogation over 935,000 methylation sites quantitatively across the genome at single-nucleotide resolution.
epigenetics illumina dna array infinium CpG methylationAdditional compute intensive nanopore raw data processing services provided by NGI
methylation base modifications basecalling fast5