Nanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
assembly long-read nanoporeNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
long-read nanopore assemblyNanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.
long-read nanopore assemblyPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
sv smrt assembly pacbio methylation amplicon sequel hifi clr de novo iso seqNGI can generate high quality assemblies using IPA and hifiasm assemblers
pacbio hifi hifiasm ipa hic omnic scaffolding assembly