Production of high-quality proximity ligation libraries, using two restriction enzymes.
epigenetics TADs illumina de novo chromatin scaffolding library preparationA proximity-ligation protocol using a sequence-independent endonuclease, generating data for TAD identification and scaffolding.
library preparation epigenetics TADs illumina de novo chromatin scaffoldingPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
smrt assembly pacbio methylation amplicon sequel hifi clr de novo iso seq sv