NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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Associated Keywords: RNA-Seq

Illumina TruSeq Stranded mRNA

RNA sequencing of mRNAs selected through poly-A enrichment.

Illumina TruSeq Stranded total RNA (RiboZero)

RNA sequencing of all RNA in a sample after depletion of rRNA or another type of highly abundant RNA.

Illumina TruSeq Stranded RNA without selection or depletion

RNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.

Chromium Single Cell V(D)J

Simultaneous analysis of gene expression and immunoprofiling of V(D)J clonotypes in the same single cell.

Nadia instrument (Dolomite Bio)

Nadia takes scRNA-Seq to the next level by using automation and flexibility to generate high quality reproducible single cell data.

TaKaRa SMARTer pico RNA kit

The Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit is specifically designed for very low input total RNA samples. It also works with degraded total RNA.

RNA-seq analysis

Runs with illumina total RNA-sequencing data. Aligns to the reference genome, gives QC metrics and finishes with gene count matrices.