Evercode combinatorial barcoding technology to enable high throughput single-cell transcriptomics.
In split-pool barcoding methods, single-cell identity is generated through successive rounds of distributing fixed cells across plate wells, where each round introduces a distinct barcode. After each barcoding step, cells are pooled and redistributed, and the final combination of barcodes uniquely labels the cDNAs from each individual cell.
The Parse Biosciences Evercode™ split-pool combinatorial barcoding system involves multiple rounds of barcoding of fixed and permeabilised cells.
Multiple rounds of barcoding of fixed and permeabilised cells.
transcriptomics RNA mRNA illumina RNA-Seq single-cell parse combinatorial