Nimagen EasySeq Full-length 16S library preparation (ONT)

Full length sequencing of the 16S gene provides a significantly higher taxonomic resolution compared to sequencing of isolated regions.

The EasySeq Full-Length 16S Library Prep Kit (NimaGen) enables efficient preparation of libraries for Oxford Nanopore sequencing of the complete 16S rRNA gene.

The protocol can process DNA from both low-biomass and high-diversity samples, with no need for culture-based enrichment.

The EasySeq protocol uses NimaGen’s RC-PCR technology to perform targeted amplification and sample barcoding in a single reaction. The kit amplifies the full-length 16S rRNA gene (V1–V9 regions), enabling superior taxonomic classification. After library prep, samples are compatible with ONT’s ligation sequencing workflows (SQK-LSK114) for use on ONT instruments.

Total price for library preparation of 1 plate (max 93 samples) and sequencing is ca 35000 SEK.

Please contact us for more information.

Last Updated: 9th June 2026

Please read our sample submission instructions before sending samples:

Sample Submission Guidelines
Applications
Relevant Technologies
Bioinformatics Pipelines
Method Status

Service

We are routinely running this method. Please visit the Order Portal to place an order.

NGI Stockholm

This protocol is available at NGI Stockholm.

Keywords