Methods to identify and study variation within specific genomic regions of interest.
Adaptive sampling is an Oxford Nanopore Technologies (ONT) method for real-time targeted sequencing. The method enables enrichment or depletion of specific genomic regions during sequencing without additional library preparation steps.
nanopore adaptive samplingDesign targeted sequencing panels for your regions of interest. Twist Bioscience provides the probes, and NGI handles QC, library prep, and sequencing.
methylation RNA dna twist bioscienceFull length sequencing of the 16S gene provides a significantly higher taxonomic resolution compared to sequencing of isolated regions.
targeted library preparation illumina 16S ampliconSequencing of the 16S V3/V4 region to study bacterial diversity and composition in a sample.
amplicon targeted library preparation illumina 16SSequencing of PCR amplicons to study genetic variation within small target regions.
library preparation illumina 16S amplicon targetedPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
de novo iso seq sv revio smrt assembly pacbio methylation amplicon hifiGenotyping-by-sequencing without prior genome information.
library preparation genome illumina wholegenome dna polymorphism