Methods to identify and study variation within specific genomic regions of interest.
Adaptive sampling is an Oxford Nanopore Technologies (ONT) method for real-time targeted sequencing. The method enables enrichment or depletion of specific genomic regions during sequencing without additional library preparation steps.
nanopore adaptive samplingDesign targeted sequencing panels for your regions of interest. Twist Bioscience provides the probes, and NGI handles QC, library prep, and sequencing.
methylation RNA dna twist bioscienceFull length sequencing of the 16S gene provides a significantly higher taxonomic resolution compared to sequencing of isolated regions.
illumina 16S amplicon targeted library preparationSequencing of the 16S V3/V4 region to study bacterial diversity and composition in a sample.
illumina 16S amplicon targeted library preparationSequencing of PCR amplicons to study genetic variation within small target regions.
amplicon targeted library preparation illumina 16SPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
assembly pacbio methylation amplicon hifi de novo iso seq sv revio smrtGenotyping-by-sequencing without prior genome information.
wholegenome dna polymorphism library preparation genome illumina