NGI during Covid-19 outbreak

NGI is still up and running during the Covid-19 pandemic, but we are experiencing some limitations in terms of personnel and key reagents. Each NGI node is following its respective host university recommendations and will continue operation until further notice.

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SPlinted Ligation Adapter Tagging (SPLAT) for Whole Genome Bisulphite Sequencing (WGBS)

SPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNA

Our R&D unit at NGI has developed a novel approach for quick and cost-effective Whole-Genome Bisulphite Sequencing (WGBS) library preparation. Compared with currently used commercial methods, this method called  SPLinted Adaptor Tagging (SPLAT) has the major advantage of uniform coverage of the genome. SPLAT is based on initial bisulphite treatment of the DNA, followed by adaptor tagging of single stranded DNA fragments, and enables WGBS using low quantities of the DNA samples.

The article presenting this method, including a detailed laboratory protocol, was published online in Nucleic Acids Research on November 28, 2016. The article presents validation of SPLAT against three commonly used WGBS library preparation techniques, two of which are based on bisulphite treatment prior to adapter tagging and one is a conventional WGBS method. Read the article to learn more.

For more information regarding this technique, please contact our project coordinators.

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Method Status

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We are routinely running this method. Please visit the Order Portal to place an order.

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