Methods to determine the proportion of Cytosine methylation (5mC), the most common type of base modification in DNA.
NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.methylation epigenetics illumina CpG WGBS EM-seq library preparation
SPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNAssDNA library preparation methylation epigenetics illumina CpG WGBS Bisulphite single-stranded DNA
Nanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.nanopore assembly long-read
PacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.sequel hifi clr de novo iso seq sv smrt assembly pacbio methylation amplicon
The EPIC BeadChip array allows for the interrogation over 935,000 methylation sites quantitatively across the genome at single-nucleotide resolution.CpG methylation epigenetics illumina dna array infinium
Runs with methylation sequencing data. It pre-processes raw data from FastQ inputs, aligns the reads and performs extensive quality-control on the results, using either Bismark or bwa-meth/MethylDackel.Bisulfite methylseq Methyl-Seq BS