Methods to determine the proportion of Cytosine methylation (5mC), the most common type of base modification in DNA.
NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.library preparation methylation epigenetics illumina CpG WGBS EM-seq
SPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNAWGBS Bisulphite single-stranded DNA ssDNA library preparation methylation epigenetics illumina CpG
PacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.smrt assembly pacbio methylation amplicon sequel hifi clr de novo iso seq sv