RNA sequencing methods that can be used to annotate de-novo genomes with transcript locations.
RNA sequencing of mRNAs selected through poly-A enrichment.
RNA-Seq library preparation transcriptomics RNA truseq mRNA illuminaNanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
assembly long-read nanoporeNanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.
nanopore assembly long-readPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
assembly pacbio methylation amplicon hifi de novo iso seq sv revio smrt