RNA sequencing methods that can be used to annotate de-novo genomes with transcript locations.
RNA sequencing of mRNAs selected through poly-A enrichment.illumina RNA-Seq library preparation transcriptomics RNA truseq mRNA
Nanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.assembly long-read nanopore
Nanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.long-read nanopore assembly
PacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.smrt assembly pacbio methylation amplicon sequel hifi clr de novo iso seq sv