Production of high-quality proximity ligation libraries, using two restriction enzymes.
illumina de novo chromatin scaffolding library preparation epigenetics TADsA proximity-ligation protocol using a sequence-independent endonuclease, generating data for TAD identification and scaffolding.
de novo chromatin scaffolding library preparation epigenetics TADs illuminaNGI can generate high quality assemblies using IPA and hifiasm assemblers
hic omnic revio scaffolding assembly pacbio hifi hifiasm ipa