This protocol is available at both NGI nodes.
RNA sequencing of mRNAs selected through poly-A enrichment.
library preparation transcriptomics RNA truseq mRNA illumina RNA-SeqRNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
library preparation RNA truseq illumina rnaseq RNA-SeqNGI can sequence user-prepared libraries on all Illumina instruments. User-prepared libraries orders can only be whole-lane orders. Libraries with custom setups and or custom primers can only be whole flow cells.
illuminaLibrary preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.
dna library preparation genome illumina WGSNanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
long-read nanopore assemblyNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
assembly long-read nanoporeNanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.
nanopore assembly long-read