G‑SPLAT: Genomic SPLAT for Whole Genome Sequencing

G-SPLAT is a library preparation technique designed for low-quality or limited-input DNA, with applications in whole-genome sequencing, metagenomics, and and other challenging sequencing projects.

G‑SPLAT (Genomic SPLinted Ligation Adapter Tagging) is a streamlined whole genome sequencing (WGS) library preparation method developed by our R&D team at NGI Sweden. It is directly adapted from our BSK‑SPLAT protocol, which is used for whole genome bisulfite sequencing (WGBS), but in this version the bisulfite conversion step is omitted. By retaining the efficient adapter ligation strategy for ssDNA (or denatured dsDNA), G-SPLAT is optimized for a wide range of challenging DNA samples, including single-stranded, double-stranded, degraded, or low-input material. Standard DNA input is 10–200 ng; however, inputs as low as 1 ng are feasible for this method. Its performance is on par with commercial kits, but it is particularly advantageous for single-stranded DNA, where alternative methods often fail.

Key Features

• Same SPLAT ligation chemistry, adapted for genomic DNA
• Ideal for difficult samples, including for example: FFPE, metagenomic samples, eDNA, cell free DNA, ssDNA-viruses.
• Useful for fragmentomics as it preserves native ends
• Cost-efficient and reproducible like BSK‑SPLAT

G‑SPLAT is a cost efficient, in-house alternative for whole-genome sequencing (WGS) when working with challenging DNA, offering flexibility, high performance, and reliable results. For more information regarding this technique, please contact our project coordinators.

Last Updated: 14th August 2025