This protocol is available at NGI Uppsala.
Method for multiplex protein biomarker analysis (5400 proeins) with sequencing readout using the Illumina NovaSeq platform.
protein Olink biomarker Olink HTMethod for multiplex protein biomarker analysis with sequencing readout using the Illumina NovaSeq platform.
protein Olink biomarker Olink ExploreNEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
library preparation methylation epigenetics illumina CpG WGBS EM-seqTotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
library preparation transcriptomics RNA mRNA illumina RNA-SeqNGI now offers High Molecular Weight (HMW) DNA extraction as a service for sequencing projects at or in collaboration with NGI and SciLifeLab.
dna extraction hmw hmw dnaSPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNA
illumina CpG WGBS Bisulphite single-stranded DNA ssDNA library preparation methylation epigeneticsGold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.
genome illumina WGS dna library preparation truseqLibrary preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.
genome illumina WGS dna library preparation truseqPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
revio smrt assembly pacbio methylation amplicon hifi de novo iso seq svA platform for human whole exome sequencing (WES) using target enrichment and library preparation for next generation sequencing.
illumina dna exome targeted library preparation