Methods for the initial sequencing of genomic DNA in order to build a draft genome reference.
Low cost library preparation option for gDNA based on bead-linked transposase. Only for full plates of samples.
genome illumina WGS dna nextera normalization library preparationMethod for shotgun DNA libraries used for whole genome sequencing and metagenomics.
tagmentation PCR-free library preparation genome illumina WGS dnaGold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.
genome illumina WGS dna library preparation truseqLibrary preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.
genome illumina WGS dna library preparation truseqLibrary preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.
genome illumina WGS dna library preparationNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
iso seq sv smrt assembly pacbio methylation amplicon sequel hifi clr de novoNGI can generate high quality assemblies using IPA and hifiasm assemblers
pacbio hifi hifiasm ipa hic omnic scaffolding assembly