Methods for the initial sequencing of genomic DNA in order to build a draft genome reference.
G-SPLAT is a library preparation technique designed for low-quality or limited-input DNA, with applications in whole-genome sequencing, metagenomics, and and other challenging sequencing projects.
dna single-stranded DNA ssDNA library preparation genome illumina WGSLow cost library preparation option for gDNA based on bead-linked transposase. Only for full plates of samples.
illumina WGS dna nextera normalization library preparation genomeMethod for shotgun DNA libraries used for whole genome sequencing and metagenomics.
tagmentation PCR-free library preparation genome illumina WGS dnaGold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.
WGS dna library preparation truseq genome illuminaLibrary preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.
genome illumina WGS dna library preparation truseqLibrary preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.
illumina WGS dna library preparation genomeNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
long-read nanopore assemblyPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
revio smrt assembly pacbio methylation amplicon hifi de novo iso seq svNGI can generate high quality assemblies using IPA and hifiasm assemblers
pacbio hifi hifiasm ipa hic omnic revio scaffolding assembly