Assembly

Methods for the initial sequencing of genomic DNA in order to build a draft genome reference.

Illumina DNA

Low cost library preparation option for gDNA based on bead-linked transposase. Only for full plates of samples.

Illumina DNA PCR-Free

Method for shotgun DNA libraries used for whole genome sequencing and metagenomics.

Illumina TruSeq DNA PCR-free

Gold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.

Illumina TruSeq DNA Nano

Library preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.

SMARTer ThruPLEX DNA-seq

Library preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.

Nanopore DNA sequencing

Nanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.

PacBio SMRT sequencing

PacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.

Genome assemblies with HiFi data

NGI can generate high quality assemblies using IPA and hifiasm assemblers