Methods for the initial sequencing of genomic DNA in order to build a draft genome reference.
Low cost library preparation option for gDNA based on bead-linked transposase. Only for full plates of samples.
normalization library preparation genome illumina WGS dna nexteraMethod for shotgun DNA libraries used for whole genome sequencing and metagenomics.
genome illumina WGS dna tagmentation PCR-free library preparationGold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.
library preparation truseq genome illumina WGS dnaLibrary preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.
library preparation truseq genome illumina WGS dnaLibrary preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.
library preparation genome illumina WGS dnaNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
hifi clr de novo iso seq sv revio smrt assembly pacbio methylation amplicon sequelNGI can generate high quality assemblies using IPA and hifiasm assemblers
scaffolding assembly pacbio hifi hifiasm ipa hic omnic