Full-length sequencing of RNA transcripts can be used to investigate splicing isoforms and fusion-gene transcripts, as well as annotation of de novo genomes.
Nanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
assembly long-read nanoporeNanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
methylation amplicon sequel hifi clr de novo iso seq sv revio smrt assembly pacbioWith long and accurate HiFi reads, you can characterize the full diversity of the transcriptome
iso-seq hifi pacbio