Full-length sequencing of RNA transcripts can be used to investigate splicing isoforms and fusion-gene transcripts, as well as annotation of de novo genomes.
Loop genomics can provide both transcript counting and phasing for full length mRNA using short-reads on Illumina sequencers
library preparation RNA full length transcripts linked-reads illuminaNanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
assembly long-read nanoporeNanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
assembly methylation smrt pacbio amplicon sequel hifi clr de novo iso seq svAnalysis applications provided by NGI using PacBio’s open-source SMRT Analysis software suite.
assembly structural variation base modifications pacbio iso-seq ccs hifi resequencing