Full-length sequencing of RNA transcripts can be used to investigate splicing isoforms and fusion-gene transcripts, as well as annotation of de novo genomes.
Loop genomics can provide both transcript counting and phasing for full length mRNA using short-reads on Illumina sequencers
full length transcripts linked-reads illumina library preparation RNANanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
long-read nanopore assemblyNanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
sequel hifi clr de novo iso seq sv smrt assembly pacbio methylation ampliconWith long and accurate HiFi reads, you can characterize the full diversity of the transcriptome
pacbio iso-seq hifi