Full-length sequencing of RNA transcripts can be used to investigate splicing isoforms and fusion-gene transcripts, as well as annotation of de novo genomes.
Combining short & long reads at the single cell level.
10x Genomics full transcript library preparation single cell long-read illumina ont chromiumNanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
long-read nanopore assemblyNanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.
nanopore assembly long-readPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
assembly pacbio methylation amplicon hifi de novo iso seq sv revio smrtWith long and accurate HiFi reads, you can characterize the full diversity of the transcriptome
hifi kinnex pacbio iso-seq