Methods to identify and study variation within specific genomic regions of interest.
The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.
library preparation nanoporeSequencing of the 16S gene to study bacterial diversity and composition in a sample.
illumina 16S amplicon targeted library preparationSequencing of PCR amplicons to study genetic variation within small target regions.
amplicon targeted library preparation illumina 16SPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
smrt assembly pacbio methylation amplicon hifi de novo iso seq sv revioGenotyping-by-sequencing without prior genome information.
illumina wholegenome dna polymorphism library preparation genome