Production of high-quality proximity ligation libraries, using two restriction enzymes.
illumina de novo chromatin scaffolding library preparation epigenetics TADsA proximity-ligation protocol using a sequence-independent endonuclease, generating data for TAD identification and scaffolding.
de novo chromatin scaffolding library preparation epigenetics TADs illuminaPacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
methylation amplicon hifi de novo iso seq sv revio smrt assembly pacbio