Methods to scaffold contigs together and correct genome assembly errors.
Production of high-quality proximity ligation libraries, using two restriction enzymes.
library preparation epigenetics TADs illumina de novo chromatin scaffoldingA proximity-ligation protocol using a sequence-independent endonuclease, generating data for TAD identification and scaffolding.
epigenetics TADs illumina de novo chromatin scaffolding library preparationNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
smrt assembly pacbio methylation amplicon hifi de novo iso seq sv revio