Droplet-based single cell sequencing using the 10X Chromium platform.
The 10X Genomics Chromium single cell gene expression protocols allow one to look at gene expression in thousands of cells from each sample. The Chromium setup is based on a single cell/nucleus suspension being pushed through a channel, where it meets up with a 10X barcoded gel bead. This results in the formation of a unique GEM partition – an oil bubble where RT and cDNA synthesis is performed – and all cDNA from a particular GEM will share the same 10X barcodes. An Illumina-compatible library is made from the cDNA and sequencing is typically performed on the Illumina NextSeq or NovaSeq platforms.
Combining short & long reads at the single cell level.
long-read illumina ont chromium 10x Genomics full transcript library preparation single cellProfiling of chromatin accessibility at the single cell level.
ATAC-seq dna chromium chromatin 10x Genomics ATAC library preparation epigenetics single cell illuminaProfiling of 3´gene expression and chromatin accessibility in the same cell.
transcriptomics single cell RNA mRNA illumina ATAC-seq RNA-Seq ATAC chromium library preparation 10x GenomicsProfiling of gene expression levels at single-cell resolution.
illumina RNA-Seq chromium 10x Genomics library preparation transcriptomics single cell RNA mRNASingle-cell profiling of gene expression levels on fixed cells/nuclei.
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