Single cell sequencing using the 10X Chromium platform.
The 10X Genomics Chromium single cell gene expression protocols allow one to look at gene expression (GE) in thousands of cells from each sample. The Chromium setup is based on a single cell/nucleus suspension being pushed through a channel, where it meets up with a 10X barcoded gel bead. This results in the formation of a unique GEM partition - an oil bubble where RT and cDNA synthesis is performed - and all cDNA from a particular GEM will share the same 10X barcodes. An Illumina-compatible library is made from the cDNA and sequencing is typically performed on the Illumina NextSeq or NovaSeq platforms.
Single-cell profiling of gene expression levels on fixed cells.
RNA mRNA illumina RNA-Seq chromium 10x Genomics fixed cells library preparation transcriptomics single cellProfiling of gene expression levels at single-cell resolution.
library preparation transcriptomics single cell RNA mRNA illumina RNA-Seq chromium 10x GenomicsProfiling of chromatin accessibility at the single cell level.
epigenetics single cell illumina ATAC-seq dna chromium chromatin 10x Genomics ATAC library preparationProfiling of 3´gene expression and chromatin accessibility in the same cell.
ATAC-seq RNA-Seq ATAC chromium library preparation 10x Genomics transcriptomics single cell RNA mRNA illuminaCombining short & long reads at the single cell level.
single cell long-read illumina ont chromium 10x Genomics full transcript library preparation